The standard micro-aerophilic recipe performs one cycle, which is composed of two phases: the evacuation phase and the replacement phase.

The standard micro-aerophilic recipe composes optimum incubation results for micro-aerophilic organisms, such as for instance Campylobacter jejuni.
Initially there is 21% oxygen in the atmosphere in the jar, the same percentage as normally in the air. For micro-aerophilic conditions 6% oxygen is desired. This means 100 - (6/21 * 100) = 71,42 % of the initial atmosphere has to be replaced with an oxygen free gas mixture. To reach this, the standard micro-aerophilic program evacuates until 297 mbar, then calculates the end filling point (approximately 1040 mbar) and replaces with the connected oxygen free gas mixture. During this procedure the Oxygen concentration in the air is rarefied to 6%.
For information about different gas mixtures or concentrations in the jars, please view the information under Information - Hotline - Programming user defined atmospheres and under Products - Options. |